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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Campylobacter jejuni 11168 wild-type and Cj1608 deletion mutant under microaerophilic and paraquat stress conditions

ABSTRACT: We performed transcriptome (RNA-seq) analyses for Campylobacter jejuni 11168 wild-type (WT) and Cj1608 deletion mutant (ΔCj1608::aphA-3) under oxidative stress and optimal microaerobic growth conditions. The expression of 231 genes was affected by oxidative stress in stressed wild-type cells (WTS) compared to non-stressed cells (WT). A comparison of genes expressed in the ΔCj1608 and WT strains under optimal growth conditions revealed 380 differently expressed genes. Moreover, transcriptional changes and overall final protein levels correlated across multiple genes. The data were validated through RT-qPCR and phenotype experiments for selected processes.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168

SUBMITTER: Mateusz Noszka

PROVIDER: E-MTAB-13650 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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