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Vibrio Cholerae CRISPR-cas PAM requirements

ABSTRACT: Plasmids were constructed harboring protospacers that are perfect targets for spacers #4 and #21 of the array, but contain NNN (all possible PAM combinations) immediately upstream of the protospacer. The plasmids were introduced into V. cholerae with or without a functional CRISPR-cas system and cells were plated on selective media. Cells were collected and the protospacer plasmids were sequenced in a high throughput manner. PAMs were counted using a custom python script

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Vibrio cholerae

SUBMITTER: Anne Stringer

PROVIDER: E-MTAB-14044 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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