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Transcription Termination and Antitermination of Bacterial CRISPR Arrays

ABSTRACT: V. cholerae A50 has a functional CRISPR-cas system with a conserved boxA sequence. Plasmids harboring protospacers that are perfect targets for each spacer of the array are introduced into wt and boxA mutant V. cholerae. After a period of growth without selection, cells are collected and the protospacer plasmids are sequenced in a high throughput manner.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Vibrio cholerae

SUBMITTER: Anne Stringer

PROVIDER: E-MTAB-9631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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