Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc

ABSTRACT: RpfB is one of five resuscitation promoting factors in Mycobacterium tuberculosis. We previously published data indicating the 5' UTR of the gene contains a regulatory riboswitch with an unknown (at the time) ligand. We overexpressed the switch in Mycobacterium smegmatis (which does not contain the rpfB gene), reasoning that overexpression of the switch would s sequester the unknown ligand, giving rise to a phenotype that would help us identify it.

INSTRUMENT(S): NextSeq 2000

ORGANISM(S): Mycobacterium smegmatis str. MC2 155

SUBMITTER: Peter Polgar

PROVIDER: E-MTAB-14260 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Project description:Riboswitch Ligand Interactions

| PRJNA1077397 | ENA

Mapping of genotype-phenotype diversity amongst clinical isolates of Mycobacterium tuberculosis by RNA-seq

Project description:Mapping of genotype-phenotype diversity amongst clinical isolates of Mycobacterium tuberculosis by RNA-seq

2013-09-19 | E-MTAB-1446 | biostudies-arrayexpress

Transcription and translation of the rpsJ, rplN and rRNA operons of the tubercle bacillus

Project description:RNA sequencing of exponentially growing Mycobacterium tuberculosis H37Rv

2015-01-23 | E-MTAB-3252 | biostudies-arrayexpress

Time course RNA-seq of Mycobacterium tuberculosis exposed to nitric oxide.

Project description:Mycobacterium tuberculosis is an intracellular human pathogen with the ability to resist and adapt to many adverse conditions it encounters upon infection. Among these, overcoming the production of nitric oxide by macrophages could be key for M. tuberculosis success. We have challenged M. tuberculosis with a sub-lethal concentration of nitric oxide and followed the transcriptomic response through RNA-seq for 48 hours.

2017-08-29 | E-MTAB-5557 | biostudies-arrayexpress

Genome-wide mapping of transcriptional start sites defines an extensive leaderless transcriptome in Mycobacterium tuberculosis

Project description:In this study we have combined RNA-seq analysis of genome-wide transcriptional start sites with regular RNA-seq to study the transcriptional landscape of Mycobacterium tuberculosis during exponential culture and growth arrest using a starvation model where exponentially growing cells are incubated in PBS for 24 hours. Three independent biological replicates were used in this experiment.

2013-11-22 | E-MTAB-1616 | biostudies-arrayexpress

ACE-Scan of the ATP DNA aptamer on 10^12 density Gen1 DNA microarray

Project description:The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on custom DNA microarrays. The aptamers tested consist of the ATP DNA aptamer, the ATP RNA aptamer, the cocaine DNA aptamer, the human alpha-thrombin DNA aptamer, and the natural add riboswitch aptamer from the pathogenic bacteria Vibrio vulnificus. Each duplexed aptamer family consists of 1000's of synthetic constructs, each formed by hybridizing the aptamer with an aptamer-complementary element (ACE) - here, ACEs consisted of various DNA oligonucleotides synthesized as a custom DNA microarray.

2017-12-20 | E-MTAB-6374 | biostudies-arrayexpress

ACE-Scan of the cocaine DNA aptamer on 10^12 Gen1 DNA microarray

2017-12-20 | E-MTAB-6377 | biostudies-arrayexpress

ACE-Scan of the ATP RNA aptamer on 10^12 Gen1 DNA microarray

2017-12-20 | E-MTAB-6376 | biostudies-arrayexpress

Mycolicibacterium smegmatis MC2 155

Project description:Transcriptome Landscape of Mycobacterium smegmatis

| PRJNA400374 | ENA

Mycolicibacterium smegmatis MC2 155

Project description:Mycobacterium smegmatis ribosomal mutant transcriptiomes

| PRJNA375121 | ENA