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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAseq of Caeca samples from conventional (CV) and germ-free (GF) chickens at 21 days of age

ABSTRACT: The gut microbiota exerts profound influence on poultry immunity and metabolism through mechanisms that yet need to be elucidated. Here we used conventional and germ-free chickens to explore the influence of the gut microbiota on transcriptomic along the gut-lung axis in poultry. Our results demonstrated a differential regulation of genes associated with innate immunity and metabolism in the caeca of germ-free birds.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Gallus gallus

SUBMITTER: RODRIGO GUABIRABA

PROVIDER: E-MTAB-14262 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNAseq of Spleen samples from conventional (CV) and germ-free (GF) chickens at 21 days of age

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Gut microbiota influences the expression of microRNA and their target mRNA in murine colonic lamina propria mononuclear cells [miRNA]

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Gut microbiota influences the expression of microRNA and their target mRNA in murine colonic lamina propria mononuclear cells [mRNA]

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Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences [RNA-Seq]

Project description:Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birth, induces metabolic alterations and affects ileal expression of genes involved in immunity. LDP that is limited to early life transiently perturbs the microbiota, which is sufficient to induce sustained effects on body composition, indicating that microbiota interactions in infancy may be critical determinants of long-term host metabolic effects. In addition, LDP enhances the effect of high-fat diet induced obesity. The growth promotion phenotype is transferrable to germ-free hosts by LDP-selected microbiota, showing that the altered microbiota, not antibiotics per se, play a causal role. These studies characterize important variables in early-life microbe-host metabolic interaction and identify several taxa consistently linked with metabolic alterations. Male and female mice were exposed to low-dose penicillin from birth. In a second experiment, microbiota from female control and LDP mice was transferred to 3-week old female germ-free mice. Livers were collected at 8 weeks of age, RNA was extracted, and transcriptional differences were measured by RNAseq.

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