RNAseq of barley leaf and roots exposed to control and drought stress conditions
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina NovaSeq 6000
ORGANISM(S): Hordeum vulgare
SUBMITTER: Agata Daszkowska-Golec
PROVIDER: E-MTAB-14320 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:Drought is a harsh abiotic stress, with plants possessing diverse strategies to survive periods of limited water resources. Although previous research has established links between strigolactone (SL) and drought, in this study, we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. We have employed a comprehensive approach integrating transcriptome, proteome, phytohormone analyses, and physiological data to unravel differences between wild-type and hvd14 plants when responding to drought.
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Project description:The aim of the study was the elucidation of drought-induced molecular events related to plant hormones occurring in crown tissue of barley, the key organ for cereal survival. Experiments involving large-scale measurements of gene expression at transcript and protein level, hormone abundance, and phenotypic variability in a set of selected barley mutants were carried out to uncover how disturbance of GAs, BRs and SLs homeostasis may affect the barley multivariate response to drought. The characterization included early stages of plant development and continued to the later stages, with observation of effects on the whole-plant architecture and yield formation. The integration of all data allowed to enrich the knowledge of regulatory networks in barley exposed to drought.
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Project description:This study was aimed at deciphering the impact of drought and heat on genome-wide gene expression in flag leaf of barley. We employed high-throughput sequencing of mRNA to identify genes that are associated with response to drought or heat and to their combination. Our study demonstrated that under combined stress, drought was the dominant factor affecting genes expression. It was also confirmed for phenotypic traits and chlorophyll fluorescence parameters. Drought- and heat-responsive genes were associated majorly with photosynthesis, abscisic acid signaling and lipids transport. Dehydrin encoding genes were found to be universal stress-responsive genes. Stress-induced genes specific to the flag leaf size were also found. This research provided novel insight into molecular mechanisms of barley flag leaf that determine drought and heat response, also during their co-occurrence.
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Project description:The aim of the study was the elucidation of drought-induced molecular events occurred in crown tissue of barley. Independent research methods allowed to identify genes (RNA-seq) and corresponding proteins (LC/MS), whose direction of regulation was the same under water deficit conditions. They may represent a promising set of genes in breeding of new varieties with increased drought tolerance. A set of genes with contrasting regulation between the genotypes with different BRs signal transduction efficiency was determined, often of fundamental role in plant development. On the other hand, several genes encoding dehydrins were expressed independently from genotype, thus being suggested as housekeeping genes. Some candidate genes on coordination of phytohormones crosstalk have been also proposed. The multidisciplinary results provided new insights into the significant relationships between gene expression, protein and phytohormone content of crown tissue under abiotic stress.
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Project description:This SuperSeries is composed of the following subset Series: GSE29566: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress in leaf tissue. GSE29567: Global gene expression analysis of cotton (Gossypium hirsutum L.) under drought stress during fibre development stages. Refer to individual Series
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Project description:We also used microarray analysis to examine transcriptomic changes under drought, identifying thousands of genes that potentially mediate drought responses in the flower, including genes encoding transcription factors that likely play crucial regulatory roles. Arabidopsis were well-watered until after just bolting (after 24 days growth with the main stem about 1 cm high) when drought treatment was started by withholding water (defined as day 0 for drought treatment). The relative soil moisture content decreased sharply and, after about 80 hours (defined as day 3 for drought treatment), the relative soil moisture content was near 35% of the soil water-holding capacity. The soil water condition was maintained by daily watering until almost all the fruits were mature and ready to harvest (about 50 days). For well-watered (control) plants, 90% of the soil water-holding capacity was maintained until tissue harvest or after seed maturation (pots were weighed and watered twice per day). Unopened flower samples were collected, from both treated and control plants, at day 0, 3, 4, 5, 10.
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