Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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The modulatory effect of visfatin (NAMPT) on the transcriptomic profile of porcine anterior pituitary cells during the peri-implantation period


ABSTRACT: Pregnancy is a unique and critical period in a female’s life during which the whole organism is mobilized and focused on ensuring the successful survival and development of the embryo. The main regulatory system responsible for the reproductive functions during both the oestrous cycle and pregnancy is the hypothalamic-pituitary-gonadal (HPG) axis. Herein we verified hypothesis assuming the modulatory effect of visfatin on the anterior pituitary transcriptome during the peri-implantation period. We analyzed samples obtained from 5 individuals (n=5). After the enzymatic tissue digestion with the use collagenase V and pancreatin cells were divided for two groups: controls [without treatment] and visfatin (100 ng/mL) treated ones. In vitro cell cultures were conducted for 24 hours.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Sus scrofa

SUBMITTER: Kamil Dobrzyn 

PROVIDER: E-MTAB-14347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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