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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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N-hydroxy-pipecolic acid mediated priming of salicylic acid signalling in Arabidopsis thaliana

ABSTRACT: N-hydroxy-pipecolic acid (NHP) primes Aarabidopsis thaliana to confer faster and stronger activation of immune responses, but how NHP interacts with other immune-inducing phytohormones is unknown. Here we investigated the crosstalk between priming phytohormone NHP and immune inducing phytohormone salicylic acid (SA). We show that pretreatment with NHP modulates the SA-inducible transcriptome, wherein some SA-expressed genes show enhanced SA-sensitivity and some show reduced SA-sensitivity. 12 day old wild type (col-0) seedlings grown on MS media were treated with 1mM NHP or water overnight, then 0.5mM SA or water for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Robert Mason

PROVIDER: E-MTAB-14631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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