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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Azelaic acid mediated priming of salicylic acid signalling in Arabidopsis thaliana

ABSTRACT: Azelaic acid (AzA) is a candidate systemic resistance inducing phloem mobile signal in Aarabidopsis thaliana, but whether it interacts with immune signalling and how is not known. Here we investigated the crosstalk between phloem signal AzA and immune inducing phytohormone salicylic acid (SA). We show that pretreatment with AzA modulates the SA-inducible transcriptome, wherein some SA-expressed genes show enhanced SA-sensitivity, but many more show reduced SA-sensitivity. 12 day old wild type (col-0) seedlings grown on MS media were treated with 1mM AzA or 5mM MES overnight, then 0.5mM SA or 5mM MES for 6 hours by immersion in 10ml of the respective chemicals. ~50 seedlings were pooled into one biological repeat, with three biological repeats total for this experiment. Seedlings were patted dry with tissue paper and frozen in liquid nitrogen for RNA extraction.

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Robert Mason

PROVIDER: E-MTAB-14632 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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