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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genotyping amplicons from SARS-CoV-2 viruses to verify the identity of PCR products

ABSTRACT: We used endpoint PCR to verify the presence of a new subgenomic RNA in SARS-CoV-2 (termed N.iORF3) and verify using nanopore sequencing that this is expressed via a newly evolved transcription regulatory sequence (TRS). We further show that this encodes a truncated C-terminal portion of Nucleocapsid, which is an antagonist of type I interferon production. Using reverse genetics-derived viruses we show N.iORF3 contributes to viral fitness during infection and observe distinct phenotypes when the Nucleocapsid coding sequence is mutated compared to when the TRS alone is ablated.

INSTRUMENT(S): GridION

ORGANISM(S): Severe acute respiratory syndrome-related coronavirus

SUBMITTER: Harriet Victoria Mears

PROVIDER: E-MTAB-14681 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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