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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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PSC co-culture time-course 15 samples single cell RNA-sequencing

ABSTRACT: The goal of this experiment is to characterize the differentiation trajectories of pancreatic stellate cells (PSC) exposed to tumour-derived signals over a 6 days time series. Co-cultures of PSC and tumour organoids were seeded in matrigel and grown for 0-6 days in basal media. Mono-cultures of PSC were used as controls. The PSCs were recovered by flow cytometry at successive time points and subjected to single cell RNA-seq profiling via the ddSeq-Surecell system.

INSTRUMENT(S): Racham server on Uppmax, NAISS, FACS Aria-III, NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER:

PROVIDER: E-MTAB-14945 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP170636

REPOSITORIES: biostudies-arrayexpress

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