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PSC co-culture day6 11 samples single cell RNA-Seq

ABSTRACT: The goal of this experiment is to characterize the different cancer associated CAF subtypes that can be induced by tumour-derived signlas in pancreatic stellate cells (PSC). Co-cultures of PSC and tumour organoids were seeded in matrigel and grown for 6 days in basal media. Mono-cultures of PSC were used as controls. The PSCs were recovered by flow cytometry and subjected to single cell RNA-seq profiling via the ddSeq-Surecell system.

INSTRUMENT(S): Racham server on Uppmax, NAISS, FACS Aria-III, NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Cedric Patthey

PROVIDER: E-MTAB-14944 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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