Liver miRNA profiling in iron deficient and overload mice
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ABSTRACT: 8-week-old wild-type 129S6/SvEvTac mice were maintained under regular iron diet (containing 330 ppm iron) or iron-enriched diet (the same standard diet supplemented with 2% carbonyl iron) for 4 weeks. 7-week-old wild-type 129S6/SvEvTac mice were maintained under iron-deficient diet (with negligible iron content) for 5 weeks. All mice were sacrificed at the age of 12 weeks. Livers were harvested and total RNA was extracted using TRIZol method followed by miRNA-enrichment using Qiagen columns. Liver RNA samples from 3 mice (female and male) from each group were sent to Exiqon for microarray analysis.
ORGANISM(S): Mus musculus
SUBMITTER: Qifang Wu
PROVIDER: E-MTAB-1520 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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