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Unknown,Transcriptomics,Genomics,Proteomics

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Human_plasma

ABSTRACT: We adapted the NextGen sequencing technology to obtain more accurate spectra of mRNA and miRNA in circulation, specifically to explore the plasma-miRNA association with colorectal cancer and ulcerative colitis.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Homo sapiens

SUBMITTER: Hong Li

PROVIDER: E-MTAB-1568 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The complex exogenous RNA spectra in human plasma: an interface with human gut biota?

Wang Kai K Li Hong H Yuan Yue Y Etheridge Alton A Zhou Yong Y Huang David D Wilmes Paul P Galas David D

PloS one 20121210 12

Human plasma has long been a rich source for biomarker discovery. It has recently become clear that plasma RNA molecules, such as microRNA, in addition to proteins are common and can serve as biomarkers. Surveying human plasma for microRNA biomarkers using next generation sequencing technology, we observed that a significant fraction of the circulating RNA appear to originate from exogenous species. With careful analysis of sequence error statistics and other controls, we demonstrated that there ...[more]

PMID: 23251414

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