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Unknown,Transcriptomics,Genomics,Proteomics

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D. melanogaster gene expression profiling time course of Snail mutants

ABSTRACT: Expression profiling of Drosophila melanogaster halo[AJ] snail[Df(2L)TE116GW11] and halo[AJ] sna[V2] homozygous mutant embryos at two timepoints of embryogenesis. Embryos were manually sorted and assayed at cellularisation stage (stage 5 to early stage 6) and at gastrulation stage (late stage 6 to early stage 8). Homozygous mutant embryos were sorted based on their reduced transparency caused by the recessive mutation of halo[AJ]. Stage-matched halo[AJ] embryos served as wildtype control. Total RNA was extracted, amplified and hybridized to Affymetrix GeneChip Drosophila Genome array version 2. Three biological replicates at each condition were generated.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-MTAB-1598 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A conserved role for Snail as a potentiator of active transcription.

Rembold Martina M Ciglar Lucia L Yáñez-Cuna J Omar JO Zinzen Robert P RP Girardot Charles C Jain Ankit A Welte Michael A MA Stark Alexander A Leptin Maria M Furlong Eileen E M EE

Genes & development 20140108 2

The transcription factors of the Snail family are key regulators of epithelial-mesenchymal transitions, cell morphogenesis, and tumor metastasis. Since its discovery in Drosophila ∼25 years ago, Snail has been extensively studied for its role as a transcriptional repressor. Here we demonstrate that Drosophila Snail can positively modulate transcriptional activation. By combining information on in vivo occupancy with expression profiling of hand-selected, staged snail mutant embryos, we identifie ...[more]

PMID: 24402316

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