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BLIMP1 ChIPseq

ABSTRACT: As a part of a modelling experiment for transcriptional control of mouse primordial germ cell specification, the transcription factor BLIMP1 was transiently expressed in the mouse p19 embryonal carcinoma cell line and its genome wide binding sites were defined using ChIPseq.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Mus musculus

SUBMITTER: Erna Magnusdottir

PROVIDER: E-MTAB-1599 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A tripartite transcription factor network regulates primordial germ cell specification in mice.

Magnúsdóttir Erna E Dietmann Sabine S Murakami Kazuhiro K Günesdogan Ufuk U Tang Fuchou F Bao Siqin S Diamanti Evangelia E Lao Kaiqin K Gottgens Berthold B Azim Surani M M

Nature cell biology 20130714 8

Transitions in cell states are controlled by combinatorial actions of transcription factors. BLIMP1, the key regulator of primordial germ cell (PGC) specification, apparently acts together with PRDM14 and AP2γ. To investigate their individual and combinatorial functions, we first sought an in vitro system for transcriptional readouts and chromatin immunoprecipitation sequencing analysis. We then integrated this data with information from single-cell transcriptome analysis of normal and mutant PG ...[more]

PMID: 23851488

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