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Overexpression of wnt8b and dkk1b in zebrafish empryos

ABSTRACT: To investigate its role during zebrafish development, wnt signalling was either activated by the conditional expression of wnt8b or inhibited by the conditional expression of dickkopf-1b (dkk1b) under the control of the hsp70 heat shock promoter. The transgenic constructs contained a gfp reporter fusion to differentiate between transgenic embryos and their wild type siblings. Transgenic embryos and wild type control embryos at the 48 hours post fertilization stage (hpf) were exposed to heat shock (37 C) for 2 hours and were collected 4 hours after heat shock. The transcriptional response to activated or inhibited wnt signalling was profiled using microarrays and compared to wild type expression.

ORGANISM(S): Danio rerio

SUBMITTER: Georg Otto

PROVIDER: E-MTAB-1887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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