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Chromatin immunoprecipitation of TBP, NC2alpha, NC2beta, Mot1p, Spt20p, Taf1p and Rpb3p in yeast grown at 4% and shifted to 0.1% glucose

ABSTRACT: ChIP-chip of TBP, NC2alpha, NC2beta, Mot1p, Spt20p, Taf1p and Rpb3p of cells grown at 4% and shifted to 0.1% glucose.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Folkert Van Werven

PROVIDER: E-MTAB-21 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome.

van Werven Folkert J FJ van Bakel Harm H van Teeffelen Hetty A A M HA Altelaar A F Maarten AF Koerkamp Marian Groot MG Heck Albert J R AJ Holstege Frank C P FC Timmers H Th Marc HT

Genes & development 20080814 17

Promoter recognition by TATA-binding protein (TBP) is an essential step in the initiation of RNA polymerase II (pol II) mediated transcription. Genetic and biochemical studies in yeast have shown that Mot1p and NC2 play important roles in inhibiting TBP activity. To understand how TBP activity is regulated in a genome-wide manner, we profiled the binding of TBP, NC2, Mot1p, TFIID, SAGA, and pol II across the yeast genome using chromatin immunoprecipitation (ChIP)-chip for cells in exponential gr ...[more]

PMID: 18703679

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