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N-terminomic analysis of RPE secretome

ABSTRACT: Identification of HtrA1 substrate in the context of RPE secretome

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Chia-yi Chen

LAB HEAD: Oliver Schilling

PROVIDER: PXD007139 | Pride | 2018-04-09

REPOSITORIES: Pride

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Publications

N-Terminomics identifies HtrA1 cleavage of thrombospondin-1 with generation of a proangiogenic fragment in the polarized retinal pigment epithelial cell model of age-related macular degeneration.

Chen Chia-Yi CY Melo Esther E Jakob Peter P Friedlein Arno A Elsässer Brigitta B Goettig Peter P Kueppers Verena V Delobel Frederic F Stucki Corinne C Dunkley Tom T Fauser Sascha S Schilling Oliver O Iacone Roberto R

Matrix biology : journal of the International Society for Matrix Biology 20180320

Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in the elderly population. Variants in the HTRA1-ARMS2 locus have been linked to increased AMD risk. In the present study we investigated the impact of elevated HtrA1 levels on the retina pigment epithelial (RPE) secretome using a polarized culture system. Upregulation of HtrA1 alters the abundance of key proteins involved in angiogenesis and extracellular matrix remodeling. Thrombospondin-1, an angiogenesis mo ...[more]

PMID: 29572155

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