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Transcription profiling by array of mouse islet of Langerhans with FoxA1/A2 knock outs

ABSTRACT: RNAs were extracted from 10 week adult control and FoxA1/A2 Pdx1CreERT2 mice. FoxA1 and FoxA2 are ablated in adult mouse Beta cells by tamoxifen adminstration. We aim to study the roles of FoxA1 and FoxA2 on islet gene expression when they are knocked down.

ORGANISM(S): Mus musculus

SUBMITTER: Nan Gao

PROVIDER: E-MTAB-219 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Foxa1 and Foxa2 maintain the metabolic and secretory features of the mature beta-cell.

Gao Nan N Le Lay John J Qin Wei W Doliba Nicolai N Schug Jonathan J Fox Alan J AJ Smirnova Olga O Matschinsky Franz M FM Kaestner Klaus H KH

Molecular endocrinology (Baltimore, Md.) 20100609 8

Foxa1 and Foxa2 play both redundant and distinct roles in early pancreas development. We demonstrate here that inducible ablation of both transcription factors in mature mouse beta-cells leads to impaired glucose homeostasis and insulin secretion. The defects in both glucose-stimulated insulin secretion and intracellular calcium oscillation are more pronounced than those in beta-cells lacking only Foxa2. Unexpectedly, in contrast to the severe reduction of beta-cell-enriched factors contributing ...[more]

PMID: 20534694

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