Genome-wide localisation map of ScRad9-9Myc overexpressed in pYX142 grown in SC (synthetic complete medium) along with BCS and BPS copper/iron chelators
Ontology highlight
ABSTRACT: This experiment was performed in order to assess the specificity of Rad9 binding to S. cerevisiae genome. In another ChIP-chip experiment in SC BCS BPS growth conditions we have found Rad9 present to a significant number of genomic loci and wanted to examine how specific this localisation is. For that we inserted Rad9 gene in the high copy plasmid pYX142 and transformed it in BJ5457 rad9delta yeast cells. Cells were grown under the same conditions and ChIP-chip was performed. By this experiment we aimed to examine quantitavely and qualitatively the Rad9-targets when Rad9 is overexpressed and to compare the findings with previous experiments of ours.
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Christos Andreadis
PROVIDER: E-MTAB-2200 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA