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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Alexandraki-S.cerevisiae-WT SC BCS BPS

ABSTRACT: Reference experiment of wild type Saccharomyces cerevisiae cells of the BJ5457 background. Cells were grown in synthetic complete medium (SC) with the addition of the necessary aminoacids and factors (leucine, uracil, histidine, tryptophane) and harvested in exponential phase (OD=0.7-1). Cells were grown in the presence of BCS and BPS chelators.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Christos Andreadis

PROVIDER: E-MEXP-3838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Rad9 interacts with Aft1 to facilitate genome surveillance in fragile genomic sites under non-DNA damage-inducing conditions in S. cerevisiae.

Andreadis Christos C Nikolaou Christoforos C Fragiadakis George S GS Tsiliki Georgia G Alexandraki Despina D

Nucleic acids research 20141009 20

DNA damage response and repair proteins are centrally involved in genome maintenance pathways. Yet, little is known about their functional role under non-DNA damage-inducing conditions. Here we show that Rad9 checkpoint protein, known to mediate the damage signal from upstream to downstream essential kinases, interacts with Aft1 transcription factor in the budding yeast. Aft1 regulates iron homeostasis and is also involved in genome integrity having additional iron-independent functions. Using g ...[more]

PMID: 25300486

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