Monocyte to macrophage differentiation and loading/deloading with lipoproteins (eLDL, oxLDL, HDL3)
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ABSTRACT: Elutriated monocytes from 9 healthy donors with ApoE3/E3 genotype were differentiated for up to six days in vitro to macrophages using rhMCSF under serum-free conditions. Samples were taken on days 1, 4, 5 and 6 and analyzed for their transcriptomic profiles. After four days of differentiation cells were loaded for 24 hours with enzymatically modified LDL (eLDL, 40 µg/ml) or mildly oxidized LDL (oxLDL, 80 µg/ml). Finally on day five, lipid deloading was performed in serum free medium containing M-CSF and cells were incubated another 24 h with HDL3 (100 µg/mL).
INSTRUMENT(S): Agilent G2565CA Microarray Scanner System
ORGANISM(S): Homo sapiens
SUBMITTER: Stefan Wallner
PROVIDER: E-MTAB-2298 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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