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Heat shock SUMO-2 RNA-seq

ABSTRACT: RNA-seq analysis of poly-A RNA from untreated U2OS cells, and from U2OS cells exposed to heat stress (43 C) for 4 hours

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Anne Seifert

PROVIDER: E-MTAB-2349 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Proteotoxic stress reprograms the chromatin landscape of SUMO modification.

Seifert Anne A Schofield Pietà P Barton Geoffrey J GJ Hay Ronald T RT

Science signaling 20150707 384

The small ubiquitin-like modifier 2 (SUMO-2) is required for survival when cells are exposed to treatments that induce proteotoxic stress by causing the accumulation of misfolded proteins. Exposure of cells to heat shock or other forms of proteotoxic stress induces the conjugation of SUMO-2 to proteins in the nucleus. We investigated the chromatin landscape of SUMO-2 modifications in response to heat stress. Through chromatin immunoprecipitation assays coupled to high-throughput DNA sequencing a ...[more]

PMID: 26152697

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