Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of sperm storage organs (spermathecae) from female Anopheles gambiae mosquitoes as virgin and 24 hours after mating

ABSTRACT: Since Anopheles gambiae female mosquitoes mate only once in their lifetime, and store sperm received from males potentially for weeks, genes and pathways regulated by mating in the sperm storage organ (spermatheca) to preserve sperm function may represent new targets for vector control. The transcriptional response to mating in the spermatheca was investigated using Agilent two-color microarrays. Spermathecae from mated females were collected 24 h after mating and compared to age-matched virgins. 4 biological replicates were performed.

ORGANISM(S): Anopheles gambiae

SUBMITTER: Robert Shaw

PROVIDER: E-MTAB-2429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Anopheles gambiae female bodies from virgin mosquitoes and 2, 6 and 24 hours after mating

Project description:Changes in gene expression in whole Anopheles gambiae female bodies between virgin mosquitoes and females samples at 2h, 6h, and 24h after mating.

2009-01-01 | E-MEXP-1868 | biostudies-arrayexpress

Transcription profiling by array of Drosophila head and thorax from females mated with sex peptide null or sex peptide producing males

Project description:Comparison of females mated to males null for Sex-Peptide (SP0, Liu, H. and E. Kubli, Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster. Proc Natl Acad Sci U S A, 2003. 100(17): p. 9929-33) or to control, Sex-Peptide producing, males. Comparisons were made at 3 and 6 hours after mating, in dissected Head-Thorax body parts.

2009-08-01 | E-MEXP-2070 | biostudies-arrayexpress

Transcription profiling of Drosophila abdomen from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2074 | biostudies-arrayexpress

Mating induced transcriptional changes in the female lower reproductive tract (LRT) of Anopheles gambiae mosquitoes at three time points (3, 12 and 24 hours) post mating.

Project description:To gain better insight into the molecular link between the post-mating transcriptional and morphological changes and the behavioral and physiological shift induced by copulation in An. gambiae females, we performed an in-depth transcriptional analysis of the female lower reproductive tract (LRT), comprising the atrium and the spermatheca, to identify the molecular pathways specifically regulated in these female reproductive tissues. We analyzed three time points post mating (3, 12 and 24 hours post mating) in order to capture transcriptional changes occurring within a broad time window after copulation. For each time point, tissues were collected from mated and age-matched virgin females and the transcriptional profile compared across 4 biological replicates using Agilent one-color microarrays.

2015-02-03 | E-MTAB-3022 | biostudies-arrayexpress

MicroRNA profiling of Drosophila head and thorax from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2079 | biostudies-arrayexpress

Mating-induced transcriptional changes in the head of female of Anopheles gambiae mosquitoes at three time points (3, 12 and 24 hours) post mating

Project description:To gain insight into the molecular pathways and processes controlling the behavioral and physiological shift induced by copulation in An. gambiae, we performed an in-depth transcriptional analysis of the heads of mated females. We analyzed three time points post-mating (3, 12 and 24 hours post mating) in order to capture transcriptional changes occurring within a broad time window after copulation. For each time point, heads were collected from mated and age-matched virgin females and the transcriptional profile compared across 4 biological replicates using Agilent one-color microarrays.

2020-09-23 | E-MTAB-8733 | biostudies-arrayexpress

MicroRNA profiling of Drosophila abdomen from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2080 | biostudies-arrayexpress

The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days). In combination with an update and expansion of the seminal vesicle sperm proteome and sex-specific isotopic labeling to identify female-contributed sperm proteins, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-14 | PXD027807 | Pride

The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-male isotopic labeling to identify female-derived sperm proteins

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to identify female-derived proteins that contribute to the sperm proteome after long-term storage in the female’s sperm storage organs. In combination with whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days), as well as an expansion of the seminal vesicle sperm proteome, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-11 | PXD027802 | Pride

The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-female isotopic labeling to estimate abundances of female-derived sperm protein

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to estimate the relative abundance of female-derived sperm proteins after long-term storage in the female’s sperm storage organs. In combination with whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days), as well as an expansion of the seminal vesicle sperm proteome, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-14 | PXD027806 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data