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4C analysis to capture chromatin conformations of the Tfap2c-Bmp7 locus upon different genomic rearrangements in mice

ABSTRACT: A genomic locus tends to form a particular conformational structure in the nucleus, but it is not known how a specific structure is dictated. To understand the mechanism, we made serial genomic rearrangements (deletion and inversion) in the Tfap2c-Bmp7 locus in mice, and analyzed the chromatin conformation in these alleles by 4C. For the wild type allele, we took not only the whole embryo but also different tissues such as the heart, forebrain (lateral and medial part separately), limb buds, and we used only the whole embryos for the mutant alleles.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Taro Tsujimura

PROVIDER: E-MTAB-2488 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A discrete transition zone organizes the topological and regulatory autonomy of the adjacent tfap2c and bmp7 genes.

Tsujimura Taro T Klein Felix A FA Langenfeld Katja K Glaser Juliane J Huber Wolfgang W Spitz François F

PLoS genetics 20150108 1

Despite the well-documented role of remote enhancers in controlling developmental gene expression, the mechanisms that allocate enhancers to genes are poorly characterized. Here, we investigate the cis-regulatory organization of the locus containing the Tfap2c and Bmp7 genes in vivo, using a series of engineered chromosomal rearrangements. While these genes lie adjacent to one another, we demonstrate that they are independently regulated by distinct sets of enhancers, which in turn define non-ov ...[more]

PMID: 25569170

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