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Analysis of the role of yeast Slf1p in translation of specific mRNAs during oxidative stress response

ABSTRACT: The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p is an atypical La motif containing protein (LARP). LARPs are members of a superfamily of RNA-binding proteins conserved across eukaryotes. We performed transcriptomics and RIP-Seq analysis in order to explore the effect of Slf1 on the amount of mRNA of genes involved in the response to oxidative stress.

INSTRUMENT(S): AB SOLiD 4 System

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: David Talavera

PROVIDER: E-MTAB-2567 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The yeast La related protein Slf1p is a key activator of translation during the oxidative stress response.

Kershaw Christopher J CJ Costello Joseph L JL Castelli Lydia M LM Talavera David D Rowe William W Sims Paul F G PF Ashe Mark P MP Hubbard Simon J SJ Pavitt Graham D GD Grant Chris M CM

PLoS genetics 20150108 1

The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Slf1p and Sro9p are atypical-La motif containing proteins which are members of a superfamily of RNA-binding proteins conserved in eukaryotes. RIP-Seq analysis of these two yeast proteins identified overlapping and distinct sets of mRNA targets, including highly translated mRNAs such as those encoding ribosomal proteins. In paralell, transcriptome analysis of slf1Δ and sro9Δ mutant strains ...[more]

PMID: 25569619

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Analysis of the role of yeast Sro9p in controlling the translation of specific mRNAs

Project description:The mechanisms by which RNA-binding proteins control the translation of subsets of mRNAs are not yet clear. Sro9p is an atypical La motif containing protein (LARP). LARPs are members of a superfamily of RNA-binding proteins conserved across eukaryotes. We performed transcriptomics and RIP-Seq analysis in order to explore the role of Sro9 on controlling translation of genes in yeast.

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2016-03-31 | E-MTAB-3413 | biostudies-arrayexpress

Analysis of the effect of PUF3 deletion in mRNA translation in yeast

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