Hif1-alpha dependent splice factor expression and alternative splicing in neonatal mouse cardiomyocytes (NMC)
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ABSTRACT: Neonatal mouse cardiomyocytes (NMC) were cultured in normoxia (21% O2) or hypoxia (3% O2) with and without a lentiviral shRNA-mediated knockdown of Hif1-alpha. Total RNA was extracted from NMC using RNeasy kit, cDNA was synthesized using GeneChip WT cDNA Synthesis and Amplification kit (Affymetrix 900673) and hybridised to Affymetrix mouse high-resolution AltSplice microarrays.
ORGANISM(S): Mus musculus
SUBMITTER: Peter Mirtschink
PROVIDER: E-MTAB-2652 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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