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Gene expression profiles of Gastro-oesophageal cancer cell lines resistant to cisplatin, oxaliplatin and docetaxel

ABSTRACT: OE21, OE33 and AGS cell lines were selected for progressive resistance to oxaliplatin, cisplatin and docetaxel. Selection began at a drug dose that was 20 fold less than the IC50 concentration. Cells were grown at the same drug concentration over 4 passages and then cell viability tests performed.Drug concentrations were increased 2 to 4 - fold until the IC50 daughter/ IC50 parental ≥ 3.The panel of drug resistant cell lines generated in this way were AGSCIS5, AGSOX8, AGSDOC6, OE33CIS4, OE33OX4 and OE21OX4 with the subscript denoting the drug and final concentration of drug(μM) that cells were exposed to. Gene expression profiling was performed using Affymetrix Exon 1.0 ST Arrays ) with 3 independent replicates per cell line from three different passages

ORGANISM(S): Homo sapiens

SUBMITTER: Russell Petty

PROVIDER: E-MTAB-2860 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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