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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human Wilms tumor cells treated with insulin-like growth factor 1 receptor inhibitors

ABSTRACT: We have previously determined the insulin-like growth factor 1 receptor (IGF1R) to be amplified and overexpressed in Wilms tumour. In order to probe the efficacy and mechanisms of action of various inhibitors of the receptor, we have carried out expression profiling of Wilms tumour cells treated with 5x IC50 of the compounds PPP and NVP-AEW541 over a 24 hour time-course.

ORGANISM(S): Homo sapiens

SUBMITTER: Anita Grigoriadis

PROVIDER: E-TABM-891 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of human paediatric glioblastoma cells treated with insulin-like growth factor 1 receptor inhibitors

Project description:We have previously determined the insulin-like growth factor 1 receptor (IGF1R) to be amplified and overexpressed in paediatric glioblastoma. In order to probe the efficacy and mechanisms of action of various inhibitors of the receptor, we have carried out expression profiling of paediatric glioblastoma cells treated with 5x IC50 of the compounds PPP and NVP-AEW541 over a 24 hour time-course.

2011-01-08 | E-TABM-889 | biostudies-arrayexpress

Transcription profiling of human KNS42 cells treated with the dual pI3-kinas/mTOR inhibitor PI-103

Project description:Sensitivity to temozolomide (TMZ) is restricted to a subset of glioblastoma patients, with the major determinant of resistance a lack of promoter methylation of the gene encoding the DNA methyltransferase MGMT, although other mechanisms are thought to be active. In a genome-wide screen of paediatric and adult glioma cells, we identified a co-ordinated upregulation of HOX gene expression in the MGMT-independent cell line KNS42. As a recent study has proposed a mechanism for this observation whereby transcriptional activation of the HOXA cluster is reversible by a PI3-kinase inhibitor through an epigenetic mechanism involving histone H3K27 trimethylation, we sought to investigate whether this was active in our system. We thus treated KNS42 cells for 24 hours with the dual PI3-kinase / mTOR inhibitor PI-103 at 5x IC50 and carried out gene expression profiling using Illumina HT-12 microarrays.

2011-01-08 | E-TABM-890 | biostudies-arrayexpress

Methylation and microRNA profiling of human paediatric glioma cell lines

Project description:Constitutive signalling pathway activation is a key feature of primary tumours and cancer cell lines. The regulation of gene expression changes may be via both genomic and epigenetic means, and understanding the mechanisms by which signal transduction may be activated can provide rational targets for therapeutic intervention. We have previously carried out DNA copy and expression profiling on a unique panel of five paediatric glioma cell lines, and have noted only a limited influence of gene amplification on gene expression. In the present study we have extended our work to include a measure of global methylation changes as well as micro RNA profiling in the same cell lines. We noted various instances of signalling pathway activation associated with specific hypermethylation or miRNA regulation of gene expression at various effectors also observed in primary paediatric tumours. These data provide evidence for the multifaceted nature of gene expression changes in paediatric high grade glioma, and identify novel targets for targeted therapy in this treatment refractory disease.

2010-12-03 | E-TABM-858 | biostudies-arrayexpress

Transcription profiling by array of human untreated endometrial cancer cell lines

Project description:Gene expression array analysis of 8 untreated endometrial cancer cell lines using Illumina human WG6 version 2

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Transcription profiling of various cell types isolated from pancreatic islet grafts in Id1/Id3 deficient and wild type mice

Project description:To gain insights into the genetic program activated within the distinct vascular and inflammatory transplant microenvironments in Id1/Id3 deficient and WT mice, we performed a series of gene screening experiments using RNA from total graft tissue as well as from myeloid and endothelial (i.e., CD31+ and ISB4+) cells isolated from the grafts at 1 week post-transplantation.

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Transcription profiling of mouse B lymphocytes after thapsigargin-stimulated ER stress for 8 and 24 hours to elucidate factors that influence T cell priming

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Transcription profiling, comparative genomic hybridization, genotyping and methylation profiling by array of human breast cancer cell lines

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Transcription profiling of human fibroblast cells from punch skin biopsies obtained from 2 MACS syndrome patients and 4 ethnically matched healthy controls.

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2009-08-01 | E-TABM-735 | biostudies-arrayexpress

Transcription profiling of human 239T cells shows SUMOylation of DRIL1 directs its transcriptional activity towards leukocyte lineage-specific genes

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