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RNA-Seq of Arabidopsis thaliana developing siliques and seeds with temperature shifts

ABSTRACT: We investigated RNA involved in the maternal control of progeny seed dormancy. Basically, we grew Ler wild type plants at 22 and 16 degree Celsius before fertilization which were moved to 22 degree for seed maturation along with ft-1 plants at 22 degree through lifetime (which show decreased seed germinability). Various RNA fractions were prepared from developing siluqes (seeds removed) and seeds, and sequenced to identify genes involved in transducing maternal memory of temperature to progeny seeds.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Min Chen

PROVIDER: E-MTAB-2869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year.

Chen Min M MacGregor Dana R DR Dave Anuja A Florance Hannah H Moore Karen K Paszkiewicz Konrad K Smirnoff Nicholas N Graham Ian A IA Penfield Steven S

Proceedings of the National Academy of Sciences of the United States of America 20141216 52

Seasonal behavior is important for fitness in temperate environments but it is unclear how progeny gain their initial seasonal entrainment. Plants use temperature signals to measure time of year, and changes to life histories are therefore an important consequence of climate change. Here we show that in Arabidopsis the current and prior temperature experience of the mother plant is used to control germination of progeny seeds, via the activation of the florigen Flowering Locus T (FT) in fruit ti ...[more]

PMID: 25516986

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