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Gene expression profiling of murine T regulatory cells after insulin-like growth factor-1 stimulation

ABSTRACT: Treg cells were isolated from spleens of Foxp3cre/gfp mice, purified by FACS (CD4+ CD25+ GFP+), incubated with rhIGF-1 for two days, and further purified by FACS. RNA was prepared with the RNeasy Mini Kit according to the manufacturer’s instructions (Qiagen), followed by two rounds of RNA amplification, labelling and hybridization to M430 2.0 chips (Affymetrix).

ORGANISM(S): Mus musculus

SUBMITTER: DANIEL BILBAO

PROVIDER: E-MTAB-2951 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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