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Mass spectrometry analysis of kinetoplastid kinetochore proteins (KKT23 acetyltransferase and its interactor KKT22) and histone acetylation levels in Trypanosoma brucei

ABSTRACT: KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. Using a kinetoplastid parasite Trypanosoma brucei, here we performed immunoprecipitation and mass spectrometry of GFP-tagged KKT23 N-terminal fragment (2–150) to identify co-purifying proteins, and immunoprecipitation and mass spectrometry of H2B-GFP after KKT23 depletion to examine acetylation states of histones. We also performed in vitro acetylation assays on recombinant trypanosome nucleosomes using recombinant KKT23 protein, followed by mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Trypanosoma Brucei

SUBMITTER: Bungo Akiyoshi

LAB HEAD: Bungo Akiyoshi

PROVIDER: PXD052922 | Pride | 2024-08-19

REPOSITORIES: Pride

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			Action	DRS
	1_Note_for_PRIDE_deposition_KKT23.txt	Txt
	In_vitro_H2A_H3_Exp1_KKT23.raw	Raw
	In_vitro_H2A_H3_Exp1_control.raw	Raw
	In_vitro_H2A_H3_Exp2_KKT23.raw	Raw
	In_vitro_H2A_H3_Exp2_control.raw	Raw

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