Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA expression profile of human bone marrow-derived mesenchymal stem cells

ABSTRACT: MicroRNA expression profiling was performed in human BMSCs at 0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 7 days. RNA quantity and quality was assessed spectrophotometrically with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) with Agilent RNA 6000 Nano Kit for total RNA. The latest version of Affymetrix platform for miRNA expression analysis (Genechip miRNA 2.0 array) based on mirBase version 12 (http://www.mirbase.org/) was used to obtain miRNA profiles. Normalization and statistical analysis were performed with Partek Genomic Suite 6.6 software by means of ANOVA test. Fold-change (FC) and p-values were applied to generate miRNA differentially expressed lists.

ORGANISM(S): Homo sapiens

SUBMITTER: Jifeng Xu

PROVIDER: E-MTAB-2977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MicroRNA expression profile of human bone marrow-derived mesenchymal stem cells

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