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MicroRNA profiling by array of human skeletal muscle cells treated with TNF-alpha

ABSTRACT: Primary human skeletal muscle cells (hSkMCs) were cultured in growth medium and a fraction of dishes was switched to differentiation medium and to differentiation medium containing 2 x 103 U/ml human recombinant TNF-alpha (Roche Applied Science, Basel, Switzerland), respectively. hSkMCs cells were harvested 24 h (myoblasts day one and myotubes day one without and with 2 x 103 U/ml TNF-alpha, respectively,) after the induction of differentiation. The experiments were performed in triplicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Swanhild Meyer

PROVIDER: E-MTAB-299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study.

Meyer Swanhild U SU Kaiser Sebastian S Wagner Carola C Thirion Christian C Pfaffl Michael W MW

PloS one 20120618 6

<h4>Background</h4>Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations. Thus, we investigated the impact of seven different normalization methods (reference gene index, global geometric mean, quantile, invariant selection, loess, loessM, and generalized procrustes analysis) on intra- and inter-platform performance ...[more]

PMID: 22723911

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