Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

MicroRNA profiling by array of mouse skeletal muscle cells treated with TNF-alpha or IGF-I

ABSTRACT: Murine skeletal muscle cells pmi28 were cultured in growth medium (HamM-^Rs F10, supplemented with 20 % FCS, 2 mM L-glutamine, and 1 % Penicillin / Streptomycin). Myoblasts were cultured on laminin-1 coated dishes for an additional 24 h before switching a fraction of dishes to differentiation medium (DMEM medium containing 2 % horse serum, 2 mM L glutamine, and 1 % Penicillin / Streptomycin) and to differentiation medium containing either 2 x 103 U/ml mouse recombinant TNF-? (Roche Applied Science), or 5 M-5g/ml mouse recombinant IGF-I (Sigma-Aldrich), or carrier only. pmi28 cells (TNF-? and IGF-I treatments as well as controls) were harvested 24 h after the induction of differentiation. The experiments were performed in quadruplicates or triplicates, respectively.

ORGANISM(S): Mus musculus

SUBMITTER: Swanhild Meyer

PROVIDER: E-MTAB-1114 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study.

Meyer Swanhild U SU Kaiser Sebastian S Wagner Carola C Thirion Christian C Pfaffl Michael W MW

PloS one 20120618 6

<h4>Background</h4>Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations. Thus, we investigated the impact of seven different normalization methods (reference gene index, global geometric mean, quantile, invariant selection, loess, loessM, and generalized procrustes analysis) on intra- and inter-platform performance ...[more]

PMID: 22723911

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Dataset Information

MicroRNA profiling by array of mouse skeletal muscle cells treated with TNF-alpha or IGF-I

Publications

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs--a comparative study.

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