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Differential gene expression in Aspergillus fumigatus induced by human platelets in vitro

ABSTRACT: Differentially expressed genes in Aspergillus fumigatus following exposure to human platelets in a timeseries experiment (15min, 30min, 1h, 3h) compared to untreated fungi as reference using customized Geniom (R) Biochips (febit) comprising 9500 genes (14051 features) were studied in order to gain a better understanding of the way platelets may affect Aspergillus spp. and contribute to antifungal host defense.

INSTRUMENT(S): Geniom RT Analyzer, Geniom

ORGANISM(S): Aspergillus fumigatus Af293

SUBMITTER: Hubert Hackl

PROVIDER: E-MTAB-3024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential gene expression in Aspergillus fumigatus induced by human platelets in vitro.

Perkhofer Susanne S Zenzmaier Christoph C Frealle Emilie E Blatzer Michael M Hackl Hubert H Sartori Bettina B Lass-Flörl Cornelia C

International journal of medical microbiology : IJMM 20150117 3

Invasive aspergillosis is characterized by vascular invasion and thrombosis. In order to determine the antifungal activity of human platelets, hyphal elongation and metabolic activity of a clinical A. fumigatus isolate were measured. Genome-wide identification of differentially expressed genes in A. fumigatus was performed after exposure to platelets for 15, 30, 60 and 180 min. Data were analyzed by gene ontology annotation as well as functional categories (FunCat) and KEGG enrichment analyses. ...[more]

PMID: 25661519

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