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High salt reduces the activation of IL-4+IL-13 stimulated macrophages

ABSTRACT: High salt (NaCl) intake is a major risk factor for cardiovascular disease; high NaCl has also been shown to be a powerful modulator of pro-inflammatory immune cell polarization. To investigate the effect of high NaCl on the polarization of M(IL-4+IL-13) macrophages we performed gene expression analysis by microarray and genome wide histone modification (H3K4me3 and H4ac) analysis by ChIP-seq of murine bone marrow derived macrophages. Macrophages were polarized with recombinant IL-4 and IL-13 in vitro in the presence or absence of an additional 40mM of NaCl in the culture medium for 24h before analysis.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Matthias Gebhardt

PROVIDER: E-MTAB-3140 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High salt reduces the activation of IL-4+IL-13 stimulated macrophages

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