Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of Leishmania mexicana promastigotes, axenic amastigotes and intracellular amastigotes


ABSTRACT: We used Illumina sequencing of poly-A selected RNA of Leishmania mexicana (WHO strain MNYC/BZ/62/M379) culture-adapted promastigotes (PRO), axenic amastigotes (AXA) and intracellular amastigotes (AMA) in mouse bone marrow derived macrophages (BMDM), 24h after infection, to map 5' and 3' ends of Leishmania transcripts and determine transcript abundances. The AMA samples were prepared from total RNA of infected macrophages thus containing a mixture of leishmanial and murine RNA transcripts. We also sequenced poly-A selected RNA from uninfected BMDMs. Three biological replicates per sample.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Leishmania mexicana

SUBMITTER: Eva Gluenz 

PROVIDER: E-MTAB-3312 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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