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A Cytoskeletal Protein Complex is Essential for Division of Intracellular Amastigotes of Leishmania mexicana (LAN1416)

ABSTRACT: Previous studies in Leishmania mexicana have identified the cytoskeletal protein KHARON as being important for both flagellar trafficking of the glucose transporter GT1 and for successful cytokinesis and survival of infectious amastigote forms inside mammalian macrophages. KHARON is located in three distinct regions of the cytoskeleton: the base of the flagellum, the subpellicular microtubules, and the mitotic spindle. To deconvolve the different functions for KHARON, we have identified two partner proteins, KHAP1 and KHAP2, that associate with KHARON. KHAP1 is located only in the subpellicular microtubules, while KHAP2 is located at the subpellicular microtubules and the base of the flagellum. Both the KHAP1 and KHAP2 null mutants are unable to execute cytokinesis but are able to traffic GT1 to the flagellum. These results confirm that KHARON assembles into distinct functional complexes and that the subpellicular complex is essential for cytokinesis and viability of disease-causing amastigotes but not for flagellar membrane trafficking.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Leishmania Mexicana

DISEASE(S): Leishmaniasis

SUBMITTER: Phillip Wilmarth

LAB HEAD: Dr. Scott M. Landfear

PROVIDER: PXD019031 | Pride | 2020-08-17

REPOSITORIES: Pride

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			Action	DRS
	LAN1405_Sequence_L_mexicana_Sanger_TriTrypDB-2.2_fixed_both.fasta	Fasta
	LAN1416_BK2-P_IP_Leismania_90min_20131028_VE.raw	Raw
	LAN1416_BK2-P_IP_Leismania_90min_20131028_VE_filtered.ms2	Other
	LAN1416_BK2-P_IP_Leismania_90min_20131028_VE_filtered.sqt	Other
	LAN1416_BK2-P_IP_Leismania_90min_20131028_VE_filtered.txt	Txt

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A Cytoskeletal Protein Complex is Essential for Division of Intracellular Amastigotes of Leishmania mexicana (LAND-483 and LAND-536)

Project description:Previous studies in Leishmania mexicana have identified the cytoskeletal protein KHARON as being important for both flagellar trafficking of the glucose transporter GT1 and for successful cytokinesis and survival of infectious amastigote forms inside mammalian macrophages. KHARON is located in three distinct regions of the cytoskeleton: the base of the flagellum, the subpellicular microtubules, and the mitotic spindle. To deconvolve the different functions for KHARON, we have identified two partner proteins, KHAP1 and KHAP2, that associate with KHARON. KHAP1 is located only in the subpellicular microtubules, while KHAP2 is located at the subpellicular microtubules and the base of the flagellum. Both the KHAP1 and KHAP2 null mutants are unable to execute cytokinesis but are able to traffic GT1 to the flagellum. These results confirm that KHARON assembles into distinct functional complexes and that the subpellicular complex is essential for cytokinesis and viability of disease-causing amastigotes but not for flagellar membrane trafficking.

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