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Transcription profiling by array of mouse PreMegE cells from conditional null Fog1 mutants

ABSTRACT: Gene expression of PreMegE cells from wild type mice was compared with those from conditional null mutants (Fog1 Floxed). Cre excision in mutant mice was induced with polyIC. PreMegE cells were isolated by FACS. Expression profiles of biological replicates were determined by hybridization to Affymetrix MoGene 1.0 ST arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Claus Nerlov

PROVIDER: E-MTAB-342 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FOG-1 and GATA-1 act sequentially to specify definitive megakaryocytic and erythroid progenitors.

Mancini Elena E Sanjuan-Pla Alejandra A Luciani Luisa L Moore Susan S Grover Amit A Zay Agnes A Rasmussen Kasper D KD Luc Sidinh S Bilbao Daniel D O'Carroll Donal D Jacobsen Sten Eirik SE Nerlov Claus C

The EMBO journal 20111108 2

The transcription factors that control lineage specification of haematopoietic stem cells (HSCs) have been well described for the myeloid and lymphoid lineages, whereas transcriptional control of erythroid (E) and megakaryocytic (Mk) fate is less understood. We here use conditional removal of the GATA-1 and FOG-1 transcription factors to identify FOG-1 as required for the formation of all committed Mk- and E-lineage progenitors, whereas GATA-1 was observed to be specifically required for E-linea ...[more]

PMID: 22068055

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