Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in Listeria- or vaccine-elicited T cells

ABSTRACT: B6 mice were immunized with either 2000 cfu of ova expressing Listeria monocytogenes (LM-ova) or 100ug whle ovalbumin adjuvanted with 50ug polyIC + 50ug anti-CD40 (FGK45). 7 days later, tetramer staining CD8+ T cells from the spleen were flow sorted to purity (~98%), the RNA isolated, reverse transcribed and the resulting cDNA analyzed by gene array MoGene-1_0-st-v1 gene expression determined in naïve (CD44lo) and day 7 post Listeria chalelneg or combined adjuvant vaccine immunization (internally referred to as "synergy").

ORGANISM(S): Mus musculus

PROVIDER: GSE112741 | GEO | 2018/04/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Next generation sequencing facilitates quantitative analysis of murine high-grade glibablastoma Transcriptomes after VEGF-A blockade In Vivo.

Project description:The goals of this study are to compare immunogenic profiling of tumor tissue from GL261 burden mice were treated with different dose VEGF-A antibody from Day10 after tumor implantation to the endpoint. The treatment doses are 0ug, 50ug, 100ug and 200ug.

2018-11-28 | GSE107423 | GEO

Transcriptional profiling of mouse LSK cells from conditional null Fog1 mutants

Project description:Gene expression of LSK cells from wild type mice was compared with those from conditional null mutants (Fog1 Floxed). Cre excision in mutant mice was induced with polyIC. LSK cells were isolated by FACS. Expression profiles of biological replicates were determined by hybridization to Affymetrix MoGene 1.0 ST arrays.

2011-08-03 | E-MTAB-343 | biostudies-arrayexpress

Transcription profiling by array of mouse PreMegE cells from conditional null Fog1 mutants

Project description:Gene expression of PreMegE cells from wild type mice was compared with those from conditional null mutants (Fog1 Floxed). Cre excision in mutant mice was induced with polyIC. PreMegE cells were isolated by FACS. Expression profiles of biological replicates were determined by hybridization to Affymetrix MoGene 1.0 ST arrays.

2012-04-08 | E-MTAB-342 | biostudies-arrayexpress

Expression data from P15 mouse testes control and Ikbkap-knockout mice

Project description:Ikbkap/Elp1 regulates genes involved in spermatogenesis. Ikbkap deficiency causes male infertility by disrupting meiotic progression. In this dataset, we include the expression data for control and Ikbkap-knockout mouse testis. We analyzed the gene expression in control and Ikbkap-knockout testes using the the Affymetrix MoGene-1_0-st-v1 platform. Three replicates per genotype.

2013-12-31 | E-GEOD-42230 | biostudies-arrayexpress

Mus musculus

Project description:Expression data from intestinal epithelial cells (IECs) [MoGene-1_0-st array]

| PRJNA247394 | ENA

Whole-genome microarray analysis of primary, secondary, tertiary and quaternary memory CD8 T cells

Project description:The transcriptome of naive OT-I T cells was compared to memory CD8 T cells after 1, 2, 3, or 4 infection with ovalbumin expressing Listeria monocytogenes (LM-OVA).

2010-07-10 | GSE21360 | GEO

Tumoral transcriptome profiling of tPTEN-/- mice.

Project description:tPTEN-/- mice display a deletion of the PTEN tumor suppressor gene specifically in T cells (cross PTEN flox/flox x lck-Cre). They develop T cell lymphoma with a primary thymic tumor and invasion of most organ at late stage of the disease. Gene profiling was performed on whole tumors (ST3 stage 3 invasive lymphoma) and normal thymocytes (wt) using Affymetrix-MoGene-1_0-st-v1 chips.

2014-02-03 | E-GEOD-39591 | biostudies-arrayexpress

Nucleosome dynamics specifies genome-wide binding of the male germ cell gene regulator CTCFL and of CTCF

Project description:This SuperSeries is composed of the following subset Series: GSE34091: Nucleosome dynamics specifies genome-wide binding of the male germ cell gene regulator CTCFL and of CTCF [Mouse430_2 Expression] GSE34092: Nucleosome dynamics specifies genome-wide binding of the male germ cell gene regulator CTCFL and of CTCF [MoGene-1_0 Expression] GSE34094: Nucleosome dynamics specifies genome-wide binding of the male germ cell gene regulator CTCFL and of CTCF [ChIP-Seq] Refer to individual Series

2012-07-24 | E-GEOD-34093 | biostudies-arrayexpress

Whole-genome microarray analysis of primary, secondary, tertiary and quaternary memory CD8 T cells

Project description:The transcriptome of naive OT-I T cells was compared to memory CD8 T cells after 1, 2, 3, or 4 infection with ovalbumin expressing Listeria monocytogenes (LM-OVA). Naive Thy1.1 OT-I T cells were adoptively transferred into Thy1.2 naive hosts prior to infection with LM-OVA. The resulting memory CD8 T cell population was again adoptively transferred into naive hosts and the recipient mice were again infected with LM-OVA. The adoptive transfer was repeated up to four times to generate memory CD8 T cells with up to four consecutive antigen stimulations. Three individual mice were analyzed for each group. For quaternary memory CD8 T cells, spleens from two to three mice were pooled for each sample. Naive OT-I T cells served as control samples. http://dx.doi.org/10.1016/j.immuni.2010.06.014

2010-07-09 | E-GEOD-21360 | biostudies-arrayexpress

Transcriptomics coupled proteomics reveals the critical signaling pathways regulating OVA antigen-specific CD8+ T cells in mouse spleen and liver

Project description:Antigen-specific T cells are particularly important for eliminating bacterial infection, and the dynamics in this process help uncover the mechanisms by which bacteria are eliminated.This study used MHC class I tetramers to recognize the antigen OVA antigen-specific CD8+ T cells in mouse spleen and liver. Then combined the RNAseq and LC-MS/MS analysis to track the changes of the OVA antigen-specific CD8+ T cells at different time points (5 days,7 days, and 14 days post listeria infection).Transcriptomics coupled proteomics reveals the key signaling pathways to regulate antigen-specific CD8+ T cells by infection of Listeria monocytogenes expressing the OVA protein in mouse spleen and liver.

2024-03-26 | PXD050970 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data