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Reprogramming of mouse gall bladder cells into pancreatic beta cells

ABSTRACT: Mouse gall bladder cells from MIP-GFP mice were expanded in vitro and transduced with adenoviral vectors expressing the transcription factors Neurog3, Pdx1 and MafA. Reprogrammed GFP+ cells were then FACS-sorted for RNA isolation.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Markus Grompe

PROVIDER: E-MTAB-3520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Generation of islet-like cells from mouse gall bladder by direct ex vivo reprogramming.

Hickey Raymond D RD Galivo Feorillo F Schug Jonathan J Brehm Michael A MA Haft Annelise A Wang Yuhan Y Benedetti Eric E Gu Guoqiang G Magnuson Mark A MA Shultz Leonard D LD Lagasse Eric E Greiner Dale L DL Kaestner Klaus H KH Grompe Markus M

Stem cell research 20130218 1

Cell replacement is an emerging therapy for type 1 diabetes. Pluripotent stem cells have received a lot of attention as a potential source of transplantable β-cells, but their ability to form teratomas poses significant risks. Here, we evaluated the potential of primary mouse gall bladder epithelial cells (GBCs) as targets for ex vivo genetic reprogramming to the β-cell fate. Conditions for robust expansion and genetic transduction of primary GBCs by adenoviral vectors were developed. Using a GF ...[more]

PMID: 23562832

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