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Genome-scale transcriptomic study into the role of AbsA2 in regulating antibiotic biosynthesis in Streptomyces coelicolor

ABSTRACT: Here we report the construction of a absA2 mutant strain which exhibited the classic precocious hyper-production of antibiotics and its complementation by an N-terminal triple-FLAG tagged version of AbsA2. The complemented and non-complemented absA2 mutant strains were used in large-scale time-course experiments to investigate the effect of deleting absA2 on gene expression at multiple time points and identify AbsA2 DNA binding sites in ChIP-on-chip studies using high-density microarrays.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Emma Laing

PROVIDER: E-MTAB-3528 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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