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Th2 differentiation in a time course experiment and at single generation resolution

ABSTRACT: Mouse splenic naïve T cells were activated (with anti-CD3/CD28)and polarized towards the Th2 subtype with IL4 . Cells were collected at different time points during the differentiation and, at day 3 .5,were collected according to number of cell divisions and expression or absence of a specific differentiation marker.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Gozde Kar

PROVIDER: E-MTAB-3543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation.

Proserpio Valentina V Piccolo Andrea A Haim-Vilmovsky Liora L Kar Gozde G Lönnberg Tapio T Svensson Valentine V Pramanik Jhuma J Natarajan Kedar Nath KN Zhai Weichao W Zhang Xiuwei X Donati Giacomo G Kayikci Melis M Kotar Jurij J McKenzie Andrew N J AN Montandon Ruddy R Billker Oliver O Woodhouse Steven S Cicuta Pietro P Nicodemi Mario M Teichmann Sarah A SA

Genome biology 20160512

<h4>Background</h4>Differentiation of lymphocytes is frequently accompanied by cell cycle changes, interplay that is of central importance for immunity but is still incompletely understood. Here, we interrogate and quantitatively model how proliferation is linked to differentiation in CD4+ T cells.<h4>Results</h4>We perform ex vivo single-cell RNA-sequencing of CD4+ T cells during a mouse model of infection that elicits a type 2 immune response and infer that the differentiated, cytokine-produci ...[more]

PMID: 27176874

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