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Transcription profiling by array of an amylolytic yeast

ABSTRACT: The amylolytic S. cerevisiae strain WTPB-G and the wild-type strain (control) from a genetic background of FY23 were used to elucidate the effect brought about to a wild-type strain by the presence of an extrachoromosomal self-replicating element in the form of a recombinant multicopy plasmid. Strains were cultivated in YPD medium in fully controlled 2 L B-Braun Biostat B Plus fermenters, with a working volume of 1.5 L under aerated and micro-aerated conditions. Samples for transcriptional profiling were collected at the mid-exponential phase of growth at an OD range of 0.7-0.9, immediately frozen in liquid nitrogen and stored at -80C until RNA isolation. All experiments were carried out in duplicates.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ceyda Kasavi

PROVIDER: E-MTAB-3545 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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