RNA-seq of Sorghum bicolor with drought tolerant genotype grown under drought-stress or no-stress control environment

ABSTRACT: The present study is expected to reveal differentially expressed genes under drought stress of Sorghum bicolor. The seeds of Sorghum genotype drought tolerant (DT) were grown at 28-32°C day/night temperature with 12/12 h light/dark period in the phytotron glass house. The fully opened uppermost leaves from control and drought stressed seedlings were sampled and stored at -80°C. For RNA-Seq libraries, one microgram of total RNA was extracted with Trizol reagent (Invitrogen, USA) and mRNA libraries were produced using the TruSeq mRNA-Seq library kit (Illumina) according to manufacturer’s instructions. The libraries generated were quantitated using an Agilent Bioanalyzer DNA 1000 chip. (Agilent Technologies, Santa Clara, CA) and a 2x101 cycle paired end sequencing (sequenced by Sandor Pvt. Lt

INSTRUMENT(S): Illumina HiScanSQ

ORGANISM(S): Sorghum bicolor

SUBMITTER: Amit Katiyar 

PROVIDER: E-MTAB-3571 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress