Proteomics

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Top-Down Mass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation

ABSTRACT: This study explores the changes in histone modifications of sorghum bicolor through developmental stages and in response to drought stress in two sorghum genotypes. We analyzed the leaves of 48 plants using top-down mass spectrometry and identified 26 unique histone proteins and 677 unique histone proteoforms. We detected trimethylation on nearly all H2B N-termini where acetylation is commonly expected. In addition, an unexpected modification on H2A histones was assigned to N-pyruvic acid 2-iminylation based on its unique neutral loss of CO2.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Sorghum Bicolor

TISSUE(S): Leaf

SUBMITTER: Matthew Monroe  

LAB HEAD: Ljiljana Pasa-Tolic

PROVIDER: PXD014660 | Pride | 2019-10-30

REPOSITORIES: Pride

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Publications

Top-down mass spectrometry of histone modifications in sorghum reveals potential epigenetic markers for drought acclimation.

Zhou Mowei M   Malhan Neha N   Ahkami Amir H AH   Engbrecht Kristin K   Myers Gabriel G   Dahlberg Jeffery J   Hollingsworth Joy J   Sievert Julie A JA   Hutmacher Robert R   Madera Mary M   Lemaux Peggy G PG   Hixson Kim K KK   Jansson Christer C   Paša-Tolić Ljiljana L  

Methods (San Diego, Calif.) 20191023

Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed leaves o  ...[more]

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