Proteomics

Dataset Information

0

Top-Down Mass Spectrometry Analysis of Histone Modifications in Sorghum during Drought Acclimation

ABSTRACT: This study explores the changes in histone modifications of sorghum bicolor through developmental stages and in response to drought stress in two sorghum genotypes. We analyzed the leaves of 48 plants using top-down mass spectrometry and identified 26 unique histone proteins and 677 unique histone proteoforms. We detected trimethylation on nearly all H2B N-termini where acetylation is commonly expected. In addition, an unexpected modification on H2A histones was assigned to N-pyruvic acid 2-iminylation based on its unique neutral loss of CO2.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Sorghum Bicolor

TISSUE(S): Leaf

SUBMITTER: Matthew Monroe  

LAB HEAD: Ljiljana Pasa-Tolic

PROVIDER: PXD014660 | Pride | 2019-10-30

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Sorghum-Histone06221162L05.raw Raw
Sorghum-Histone06221162L05_IcTsv.zip Other
Sorghum-Histone0622162L01.raw Raw
Sorghum-Histone0622162L01_IcTsv.zip Other
Sorghum-Histone0622162L02.raw Raw
Items per page:
1 - 5 of 96
altmetric image

Publications

Top-down mass spectrometry of histone modifications in sorghum reveals potential epigenetic markers for drought acclimation.

Zhou Mowei M   Malhan Neha N   Ahkami Amir H AH   Engbrecht Kristin K   Myers Gabriel G   Dahlberg Jeffery J   Hollingsworth Joy J   Sievert Julie A JA   Hutmacher Robert R   Madera Mary M   Lemaux Peggy G PG   Hixson Kim K KK   Jansson Christer C   Paša-Tolić Ljiljana L  

Methods (San Diego, Calif.) 20191023

Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed leaves o  ...[more]

Similar Datasets

Transcriptomics Sorghum nitrogen stress tolerance
2014-05-01 | GSE54705 | GEO
Transcriptomics Sorghum nitrogen stress tolerance
2014-05-01 | E-GEOD-54705 | biostudies-arrayexpress
Proteomics Sorghum seed proteome by 4D-DIA
2024-11-24 | PXD056775 | Pride
Proteomics Sorghum brewing SWATH-MS proteomics
2022-10-21 | PXD034981 | Pride
Transcriptomics Drought-responsive small RNAs in Sorghum bicolor (L.) Moench
2014-04-15 | E-MTAB-1630 | biostudies-arrayexpress
Proteomics Epigenetic Control of Drought Acclimation in Sorghum (EPICON)
2017-10-23 | MSV000081639 | MassIVE
Transcriptomics Sorghum gene expression using Agilent custom 4x44K Microarray
2012-04-08 | GSE36689 | GEO
Transcriptomics Tissue specific expression data in Sorghum bicolor BTx623
2015-03-11 | E-MTAB-4273 | biostudies-arrayexpress
Transcriptomics Sorghum gene expression using Agilent custom 4x44K Microarray
2012-04-07 | E-GEOD-36689 | biostudies-arrayexpress
Transcriptomics Effect of phosphorus deficiency on gene expression of sorghum roots
2024-10-01 | GSE245050 | GEO