Hippocampal gene expression from acyl-ghrelin treated mice
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ABSTRACT: Ghsr-/- mice and WT controls (14 weeks-old, n = 3 / group) were used to analyse whole genome gene expression in the hippocampal DG. Briefly, mice were prepared with jugular vein cannulae attached to osmotic mini-pumps (Alzet model 2001) under isofluorane anesthesia. The mini-pumps delivered either vehicle or acyl-ghrelin (48μg/day; Phoenix Pharmaceuticals, USA) for 7 days. Mice were killed by cervical dislocation and whole brain was removed and immediately snap frozen on dry ice and stored at -80oC prior to T-RNA extraction for microarray analysis.
ORGANISM(S): Mus musculus
SUBMITTER: Jeffrey Stephens Davies
PROVIDER: E-MTAB-3601 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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