Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of the Hha-dependent genes in Salmonella entrica serovar Typhimurium SL1344

ABSTRACT: Transcriptomic analysis in Salmonella enterica serovar Thypimurium SL1344 mutant strains hha, stpA and point mutant D48N compared to wild type strain

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

SUBMITTER: Cristina Madrid Xufré

PROVIDER: E-MTAB-3621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Identification of the Tetracycline dependent genes in Salmonella enterica serovar Typhimurium SL1344 (R27)

Project description:Transcriptomic analysis in Salmonella enterica serovar Thypimurium SL1344 (R27) with or without the presence of Tetracycline

2018-05-14 | E-MTAB-5774 | biostudies-arrayexpress

Growth phase-dependent transcriptional control of R27 plasmid genes

Project description:Transcriptomic analysis of R27 plasmid genes in Escherichia coli MG1655 strain grown in logarithmic and early-stationary phase

2016-11-30 | E-MTAB-4067 | biostudies-arrayexpress

Transcriptome of Salmonella enterica serovar Typhimurium in the greA, greB and greAgreB mutant strains

Project description:Transcriptomic analysis of Salmonella enterica serovar Thypimurium SL1344 mutant strains greA, greB and greAgreB compared to wild type strain

2024-01-01 | E-MTAB-13609 | biostudies-arrayexpress

Identification of Salmonella enterica genes under the control of StdE and StdF

Project description:Transcriptomic analysis in a Salmonella enterica Serovar Typhimurium SL 1344 that constitutively expresses stdE and stdF compared with a strain carrying an stdEF deletion A four chip study using total RNA recovered from two separate cultures of Salmonella enterica Serovar Typhimurium SL 1344 constitutively expressing stdE and stdF and two separate cultures of Salmonella enterica Serovar Typhimurium SL 1344 lacking stdE and stdF.

2014-11-25 | E-GEOD-45488 | biostudies-arrayexpress

Temperature-dependent transcriptional control of R27 genes

Project description:Transcriptomic analysis of R27 plasmid genes in Escherichia coli MG1655 strain grown at 25 and 37ºC

2020-05-25 | E-MTAB-9150 | biostudies-arrayexpress

ChAP-chip analysis of Hha binding regions in wild type and hnsstpA E. coli cells

Project description:The analysis of the distribution of Hha binding regions (His-Hha were expressed from pQE plasmid) on E. coli chromosome in E. coli W3110 (wild type) and hns/stpA mutant cells.

2013-04-02 | E-MEXP-3813 | biostudies-arrayexpress

Transcriptome comparison of E. coli W3110 and hns stpA hha and ydgT mutant cells

Project description:Transcriptome comparison of E.coli W3110 (wild type), hha, ydgT, hns, stpA, hha_ydgT double and hns_stpA double mutant cells

2013-04-04 | E-MEXP-3811 | biostudies-arrayexpress

Biofilm dispersal of Hha13D6 vs. Hha and biofilm formation of Hha24E9 vs. Hha in E.coli K-12 BW25113 hha mutant in LBglu at 37oC

Project description:The global transcriptional regulator Hha of Escherichia coli controls hemolysin activity, biofilm formation, and virulence expressions. Earlier, we have reported that Hha represses initial biofilm formation and disperses biofilms as well as controls prophage excision in E. coli. Since biofilm dispersal is a promising area to control biofilms, here we rewired Hha to control biofilm dispersal and formation. The Hha variant Hha13D6 was obtained to have enhanced biofilm dispersal activity along with increased toxicity compared to wild-type Hha (Hha13D6 induces dispersal 60%, whereas wild-type Hha induces dispersal at early biofilms but not at mature biofilms). Toxic Hha13D6 caused cell death probably by the activation of proteases HslUV, Lon, and PrlC, and deletion of protease gene hslV with overproducing Hh13D6 repressed biofilm dispersal, indicating Hha13D6 induces biofilm dispersal through the activity of protease HslV. Furthermore, another Hha variant Hha24E9 was also obtained to decrease biofilm formation 4-fold compared to wild-type Hha by regulation of gadW, glpT, and phnF. However, the dispersal variant Hha13D6 did not decrease biofilm formation, while the biofilm variant Hha24E9 did not induce biofilm dispersal. Hence, Hha may have evolved two ways in response to environmental factors to control biofilm dispersal and formation, but both controlling mechanisms come from different regulatory systems.

2010-10-31 | GSE21604 | GEO

E. coli K-12 hha hns: pCA24N-hnsK57N vs pCA24N-hns biofilm

Project description:The global regulator, H-NS, controls genes related to stress response, biofilm formation, and virulence expression by recognizing the curved DNA and silences gene transcription acquired from lateral gene transfer. Here, we rewired H-NS to control biofilm formation using protein engineering. One H-NS variant, H-NS K57N was obtained to reduce biofilm formation 10-fold compared to H-NS wild-type. Whole-transcriptome analysis (BW25113 hha hns / pCA24N-hns K57N vs. BW25113 hha hns / pCA24N-hns) revealed that H-NS K57N represses biofilm formation through the interactinon with other nucleoid proteins, Cnu and StpA. Remarkably, H-NS K57N enhanced the excision of defective prophage Rac while H-NS wild-type represses it, and H-NS controlled only Rac excision among E. coli prophages. These results imply that the repression of Rac excision is one of the silencing manner for foreign genes by H-NS. Also, the prophage excision not only led to the change of biofilm formation but also resulted in cell lysis through the expression of toxin protein HokD with reduced viability, which are important for cell physiology in response to the change of environmental conditions. Hence, H-NS regulatory system may be evolved easily with specialized functions in terms of biofilm formation, prophage control, and cell lysis.

2010-02-22 | GSE17853 | GEO

Expression analysis of Salmonella enterica serovar Typhimurium strain SL1344 with and without plasmid R27

Project description:Investigation of gene expression level changes in Salmonella Typhiumurium SL1344 (R27) compared to the wild-type SL1344 strain when grown at different growth temperatures and growth phases. A 24 microarray study was performed using total RNA recovered from three separate wild-type cultures of SL1344 and three separate cultures of SL1344 (R27) grown to exponential and stationary phase at 25oC and 37oC. Each microarray measured the expression level of 4,527 genes from Salmonella Typhimurium SL1344 chromosome, 103 genes from plasmid pSLT, 100 genes from plasmid pRSF, 14 genes from plasmid pCOL1B and 207 genes from plasmid R27. Seven probes were present per transcript, with two-fold technical redundancy.

2013-03-21 | E-GEOD-45347 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data